Synthesis of Nucleoside Triphosphates that Contain an Aminooxy Function for “Post‐Amplification Labelling”
Identifieur interne : 000267 ( France/Analysis ); précédent : 000266; suivant : 000268Synthesis of Nucleoside Triphosphates that Contain an Aminooxy Function for “Post‐Amplification Labelling”
Auteurs : Emmanuelle Trévisiol [France] ; Eric Defrancq [France] ; Jean Lhomme [France] ; Ali Laayoun [France] ; Philippe Cros [France]Source :
- European Journal of Organic Chemistry [ 1434-193X ] ; 2000-01.
English descriptors
- Teeft :
- Acidic conditions, Aldehydic, Aldehydic fluorophore, Aminooxy, Aminooxy function, Anhydrous, Anhydrous conditions, Anhydrous pyridine, Calcd, Cdcl3, Chem, Crude product, Deprotected nucleoside, Ether, Ethyl orthoformate, Fluorescein, Fluorescent label, Fluorophore, Full paper, Hrms, Isobutyl chloroformate, Labelling, Matrix, Methoxyamine hydrochloride, Mmol, Mnba, Mnba matrix, Nucleoside, Nucleoside triphosphates, Nucleotide, Oily residue, Orange powder, Organic layer, Oxime, Oxime ether, Pyridine, Quat, Reaction mixture, Room temp, Room temperature, Silica, Temp, Tetrahedron lett, Transcription products, Tributylammonium pyrophosphate, Triphosphate, Triphosphates, Tris buffer, Uridine, White powder.
Abstract
Preparation of the uridine and adenosine triphosphates 1 and 2 bearing a linker with a terminal aminooxy group is described. Both 1 and 2 react readily with the aldehydic fluorescein derivative 15. They could each be incorporated into a 330‐mer fragment with T7 RNA polymerase.
Url:
DOI: 10.1002/(SICI)1099-0690(200001)2000:1<211::AID-EJOC211>3.0.CO;2-V
Affiliations:
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ISTEX:9503F72893DD632AEF4F5F4F1CC1BA06E677A549Le document en format XML
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type="city">Marcy‐l'Etoile</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">European Journal of Organic Chemistry</title><title level="j" type="alt">EUROPEAN JOURNAL OF ORGANIC CHEMISTRY</title><idno type="ISSN">1434-193X</idno><idno type="eISSN">1099-0690</idno><imprint><biblScope unit="vol">2000</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="211">211</biblScope><biblScope unit="page" to="217">217</biblScope><biblScope unit="page-count">7</biblScope><publisher>WILEY‐VCH Verlag GmbH</publisher><pubPlace>Weinheim</pubPlace><date type="published" when="2000-01">2000-01</date></imprint><idno type="ISSN">1434-193X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1434-193X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acidic conditions</term><term>Aldehydic</term><term>Aldehydic fluorophore</term><term>Aminooxy</term><term>Aminooxy function</term><term>Anhydrous</term><term>Anhydrous conditions</term><term>Anhydrous pyridine</term><term>Calcd</term><term>Cdcl3</term><term>Chem</term><term>Crude product</term><term>Deprotected nucleoside</term><term>Ether</term><term>Ethyl orthoformate</term><term>Fluorescein</term><term>Fluorescent label</term><term>Fluorophore</term><term>Full paper</term><term>Hrms</term><term>Isobutyl chloroformate</term><term>Labelling</term><term>Matrix</term><term>Methoxyamine hydrochloride</term><term>Mmol</term><term>Mnba</term><term>Mnba matrix</term><term>Nucleoside</term><term>Nucleoside triphosphates</term><term>Nucleotide</term><term>Oily residue</term><term>Orange powder</term><term>Organic layer</term><term>Oxime</term><term>Oxime ether</term><term>Pyridine</term><term>Quat</term><term>Reaction mixture</term><term>Room temp</term><term>Room temperature</term><term>Silica</term><term>Temp</term><term>Tetrahedron lett</term><term>Transcription products</term><term>Tributylammonium pyrophosphate</term><term>Triphosphate</term><term>Triphosphates</term><term>Tris buffer</term><term>Uridine</term><term>White powder</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Preparation of the uridine and adenosine triphosphates 1 and 2 bearing a linker with a terminal aminooxy group is described. Both 1 and 2 react readily with the aldehydic fluorescein derivative 15. They could each be incorporated into a 330‐mer fragment with T7 RNA polymerase.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Grenoble</li><li>Marcy‐l'Etoile</li></settlement><orgName><li>Université Joseph Fourier</li></orgName></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Trevisiol, Emmanuelle" sort="Trevisiol, Emmanuelle" uniqKey="Trevisiol E" first="Emmanuelle" last="Trévisiol">Emmanuelle Trévisiol</name></region><name sortKey="Cros, Philippe" sort="Cros, Philippe" uniqKey="Cros P" first="Philippe" last="Cros">Philippe Cros</name><name sortKey="Defrancq, Eric" sort="Defrancq, Eric" uniqKey="Defrancq E" first="Eric" last="Defrancq">Eric Defrancq</name><name sortKey="Laayoun, Ali" sort="Laayoun, Ali" uniqKey="Laayoun A" first="Ali" last="Laayoun">Ali Laayoun</name><name sortKey="Lhomme, Jean" sort="Lhomme, Jean" uniqKey="Lhomme J" first="Jean" last="Lhomme">Jean Lhomme</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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